Research Paper Volume 13, Issue 23 pp 25342—25364

Figure 7. Overexpression or inhibition of hsa-miR-4535 regulates collagen synthesis in HS68 cells. (A–C) HS68 cells were transfected with hsa-miR-4535 inhibitor (40 nM) or inhibitor NC (40 nM) for 1 h and then cotreated with H2O2 (200 μM) for 23 h. (D–F) HS68 cells were transfected with hsa-miR-4535 mimic (10 nM) or mimic NC (10 nM) for 1 h and then co-treated with galangin (30 μM) for 23 h. hsa-miR-4535 levels were detected by qPCR to ensure successful transfection. Protein levels of Smad4, COL1A1, and COL3A1 were analyzed by western blotting. GAPDH was used as a loading control. Values are shown as mean ± SE. Quantification of the results is shown (n = 3) ^*P < 0.05, ^**P < 0.01, ^***P < 0.001 vs. untreated control cells; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 vs. H₂O₂ or galangin-treated cells.