Research Paper Volume 13, Issue 23 pp 25325—25341

Figure 4. Art treatment inhibits cell senescence, p^16INK4a expression and intracellular ROS production and promotes SOD expression in UVB-irradiated HaCaT cells via increasing β-catenin expression. (A, B) SA-β-gal staining for cell senescence in control group, UV group, UV + sh-NC group, UV + sh-β-catenin group, UV + sh-NC + Art group, UV + sh-β-catenin + Art group, UV + XAV-939 group and UV + XAV-939 + Art group. Scale bar: 100 μm. Magnification: 200×. (C–E) Western blot of the expression of β-catenin and p16^INK4a in HaCaT cells from each group. (F) Immunofluorescence and (G, H) Flow cytometry of intracellular ROS expression in HaCaT cells from each group. Scale bar: 100 μm. Magnification: 200×. (I) Detection of SOD levels in HaCaT cells from each group. Ns: not significant; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001.