Research Paper Volume 13, Issue 23 pp 25291—25303

Figure 1. Effect of sorbitol accumulation on oocytes of aged mice. (A) The level of sorbitol in the IVM oocytes of young mice was significantly lower than that in the GV oocytes (n = 50). (B) The level of sorbitol in aged oocytes was significantly increased after in vitro aging (n = 50). (C) Effect of in vitro maturation on the expression of AR in the oocytes of young mice. Y_GV was the GV oocyte of young mice, Y_{In vitro} was the young IVM oocyte, and Y_{In vivo} was the young oocyte during in vivo natural maturation. (D) Effect of in vitro aging on the expression of Akr1b3 in the oocytes of aged mice. Y_Aging was the oocyte of young mice undergoing in vitro aging, O_{In vivo} was the aged oocyte during in vivo natural maturation, and O_Aging was the aged oocyte undergoing in vitro aging. All experiments were repeated at least three times. ^*P < 0.05, ^**P < 0.01.