Figure 3 — MicroRNA therapy confers anti-senescent effects on doxorubicin-related cardiotoxicity by intracellular and paracrine signaling

Research Paper|Volume 13, Issue 23|pp. 25256–25270

MicroRNA therapy confers anti-senescent effects on doxorubicin-related cardiotoxicity by intracellular and paracrine signaling

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Figure 3.MiR-199a-3p directly targeted GATA4. (A) Predicted miR-199a-3p binding site in the 3’-UTR of GATA4. The corresponding sequence in the mutated (MUT) version is also shown. (B) Luciferase activity was analyzed 48 h after transfection of the miR-199a-3p mimic or miR-NC mimic. ^*P < 0.05 versus the miR-199a-3p mimic (WT group) in repeated-measures ANOVA, n = 6 per group. (C,D) Western blot analysis of GATA4. The size of markers (in kDa) is indicated. ^*P < 0.05 versus control; ^▲P < 0.05 versus Dox + miR-199a-3p mimic in repeated-measures ANOVA, n = 6 per group.