Research Paper Volume 13, Issue 23 pp 25089—25105

Figure 1. Puerarin inhibits cellular proliferation and induces mitochondrial-dependent apoptosis in PDACs. (A) The chemical structure of puerarin. (B) The growth of PDACs with or without puerarin treatment was determined by (RTCA). (C) The viability of PDACs was analyzed by the CCK-8 assay. (D) The proliferation of PDACs in different groups was analyzed by the colony formation assay. (E) Immunocytochemical staining of Ki67 in PDACs. Bar = 100 μm. (F) Protein expression of c-Myc in PDACs. (G) Flow cytometry analysis of cell apoptosis in PDACs with or without puerarin treatment. (H) Western blot analysis showing the expression of Cleaved caspase-8, Bcl-2, and Bax in PDACs. Data were presented as the mean ± standard deviation and were analyzed by one-way ANOVA with Bonferroni’s post-hoc test. ^*p < 0.05, ^**p < 0.01, and ^***p < 0.001.