Research Paper Volume 13, Issue 22 pp 24542—24559
Increased proliferation and differentiation capacity of placenta-derived mesenchymal stem cells from women of median maternal age correlates with telomere shortening
Figure 3. Chondrogenic differentiation ability of PDMSCs from different maternal age group. (A) Representative image of chondrogenic differentiation of all 5 age groups. Upper panel showed that undifferentiated cells did not retain Alcian blue staining. Lower panel showed that differentiated cells were positive to Alcian blue staining. Real Time PCR performed to measure chondrocyte markers gene expression of (B) Aggrecan (ACAN) and (C) SOX9 of all 5 age groups. Group A: 18-21; Group B: 22-25; Group C: 26-30; Group D: 31-35; Group E: 36 and over. *, p<0.05; **, p<0.01; ***, p<0.0005; ****, p<0.0001. Scale bar 200 μm.