Research Paper Volume 13, Issue 22 pp 24850—24865
Long noncoding RNA NEAT1 inhibits the acetylation of PTEN through the miR-524-5p /HDAC1 axis to promote the proliferation and invasion of laryngeal cancer cells
Figure 7. Overexpression of HDAC1 reversed the inhibitory effects of miR-524-5p overexpression and NEAT1 silence on cell proliferation and invasion. (A) The protein level of HDAC1 was detected after TU212 cells transfected with pcDNA-HDAC1 or/and miR-524-5p mimic. (B) Cell proliferation was detected with CCK-8 assay. (C) Transwell assay was used to evaluate cell invasion ability. (D) Apoptosis rates of TU212 cells. (E) The protein level of HDAC1 was detected after TU212 cells transfected with pcDNA-HDAC1 or/and si-NEAT1. (F) Cell proliferation was detected with CCK-8 assay. (G) Transwell assay was used to evaluate cell invasion ability. (H) Apoptosis rates of TU212 cells. * P < 0.05, ** P < 0.01 versus Vector and NC-mimic, Scramble and Vector, NC-mimic and pcDNA-HDAC1, and Scramble with pcDNA-HDAC1.