Research Paper Volume 13, Issue 22 pp 24850—24865
Long noncoding RNA NEAT1 inhibits the acetylation of PTEN through the miR-524-5p /HDAC1 axis to promote the proliferation and invasion of laryngeal cancer cells
Figure 4. NEAT1 promoted laryngocarcinoma growth and metastasis via sponging miR-524-5p. (A) TU212 and M2E cells were transfected si-NEAT1 or/and miR-524-5p inhibitor, and the transfection efficiency was detected using qPCR. (B, C) CCK-8 assay was performed to analyze cell proliferation. (D) Apoptosis rates in each group were detected using Flow cytometry. (E) The invasion ability was evaluated using Transwell invasion assay. (F) Western blot analysis was used to detect the protein levels of PTEN, Ac-PTEN, AKT and p-AKT. * P < 0.05, ** P < 0.01 versus Scramble and NC-inhibitor or Scramble and miR-524-5p inhibitor.