Research Paper Volume 13, Issue 22 pp 24850—24865
Long noncoding RNA NEAT1 inhibits the acetylation of PTEN through the miR-524-5p /HDAC1 axis to promote the proliferation and invasion of laryngeal cancer cells
Figure 2. Downregulation of NEAT1 suppressed laryngocarcinoma cell proliferation and invasion. (A) The transfection efficiency was detected after transfection with si-NEAT1 for 24 h in TU212 and M2E cells. (B, C) Cell proliferation of TU212 and M2E cells were detected using CCK-8 assay after transfection with si-NEAT1 for 0, 24, 48 and 72 h. (D) Apoptosis rates of TU212 and M2E cells after transfection with si-NEAT1 were detected using Flow cytometry. (E) Transwell assay was used to evaluate cell invasion ability after transfection with si-NEAT1 for 48 h. (F) Western blot analysis was used to detect the protein expression levels of PTEN, Ac-PTEN, AKT and p-AKT after transfection with si-NEAT1. ** P < 0.01 versus Scramble.