Research Paper Volume 14, Issue 1 pp 253—271
Increased expression of osteopontin in subchondral bone promotes bone turnover and remodeling, and accelerates the progression of OA in a mouse model
Figure 1. The expression of sOPN is increased in subchondral bone in OA. (A) Western blot analysis and quantification of the expression of sOPN in subchondral bone of patients with OA and control subjects. (B, C) Representative H&E, immunostaining and quantitative analysis of sOPN+ cells in the tibial subchondral bone of OA patients and control subjects. Positive cells were indicated with arrows. Scale bars = 200 μm (B), 100 μm (C), n ≥5. (D) Representative safranin O-fast green staining and OARSI scores of an OA mouse model and control group. Scale bars = 200 μm, n = 6. (E) Immunostaining and quantitative analysis of sOPN+ cells in the tibial subchondral bone of an OA mouse model and control group. Positive cells were indicated with arrows. Scale bars =50 μm, n = 6. Data are shown as mean ± s. d. and were analyzed by Student’s t test; *P < 0.05,**P < 0.01.