Research Paper Volume 13, Issue 22 pp 24605—24620
Knockdown of transient receptor potential melastatin 2 reduces renal fibrosis and inflammation by blocking transforming growth factor-β1-activated JNK1 activation in diabetic mice
Figure 2. Inflammatory response and the localization of TRPM2 in the kidneys of mice with HFD/STZ-induced diabetes. (A) qRT-qPCR assay was used to determine the mRNA expression of inflammatory factors including IL-1β, IL-6, IFN-α, TNF-α and MCP-1 in the kidney tissues of mice in the 24th week. (B) ELISA was used to determine the contents of inflammatory factors including IL-1β, IL-6, IFN-α, TNF-α and MCP-1 in the kidney tissues of mice in the 24th week. (C) qRT-PCR assay was performed to assess the mRNA levels of TRPM2 in the kidney tissues of mice in the 24th week. (D) IF double-staining analyses of TRPM2 and APQ1 in the renal tissues of mice in the 24th week. (E) qRT-PCR assay was performed to assess the mRNA levels of TRPM2 in kidney biopsies obtained from human subjects with diabetic nephropathy. The data are shown as the mean ± SD, n = 6 per group; *p < 0.05, **p < 0.01 and ***p < 0.001 versus the control group.