Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue

Ranran Zhou; Xianyuan Lv; Tianle Chen; Qi Chen; Hu Tian; Cheng Yang; Wenbin Guo; Cundong Liu

doi:doi:10.18632/aging.203675

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Research Paper Volume 13, Issue 21 pp 24219—24235

Construction and external validation of a 5-gene random forest model to diagnose non-obstructive azoospermia based on the single-cell RNA sequencing of testicular tissue

Figure 6. The diagnostic value of each variable in the random forest model. (A, B) The Mean Decrease Accuracy (A) and Mean Decrease Gini (B) of the variables. (C, D) The ROCs showed the predictive performance of each gene in the training (C) and external validation cohorts (D). (E) The expression of RPS4X in the testicular biopsy samples from 20 NOA (up) and 20 OA (down) patients (x200). ROC, receiver operating curve. AUC, area under curve; NOA, non-obstructive azoospermia; OA, obstructive azoospermia; IOD, integral optical density. *, P < 0.05.