Research Paper Volume 13, Issue 21 pp 24171—24191
LncRNA OTUD6B-AS1 promotes paclitaxel resistance in triple negative breast cancer by regulation of miR-26a-5p/MTDH pathway-mediated autophagy and genomic instability
Figure 7. The OTUD6B-AS1-miR-26a-5p-MTDH pathway mediated autophagy and DDR process. (A) IF assay displays the LC3B-I and LC3B-II focus in HCC1937 (green: LC3B-I; orange: LC3B-II; blue: DAPI), (B) WB assay detects the level of LC3B-I, LC3B-II and MTDH, (C) and IF assay displays the expression level of γ-H2AX (Green: γ-H2AX; Blue: DAPI), which are treated with recombined plasmid lncRAN-MUT or lncRNA-WT; (D) IF assay displays the LC3B-I and LC3B-II focus in HCC1937, (E) WB assay detects the level of LC3B-I, LC3B-II and MTDH, (F) and IF assay displays the expression level of γ-H2AX, which are treated with different combinations with in miRNA and PTX; (G) IF assay displays the LC3B-I and LC3B-II focus in HCC1937, (H) WB assay detects the level of LC3B-I, LC3B-II and MTDH, (I) and IF assay displays the expression level of γ-H2AX, which are treated with si-NC+PTX or si-MTDH+PTX; (J and K) WB assay detect the level of DDR process associated markers (p-ATR, ATR, p-ATM, ATM, p-RAD51, RAD51) after different treatments in HCC1937.