Figure 4.Cosmc transfection increased the activity of T-synthase and changed core 1− and core 2-derived O-glycans in Tn+ cells. (A) The activity of T-synthase was detected using a fluorescence method in LS174T-Tn−, LS174T-Tn+, and Jurkat T cells before and after transfection with WtCosmc or Mock. (B) Core 1- and core 2-derived O-glycans in Jurkat T cells before and after transfection with WtCosmc or Mock were analyzed using CORA and MALDI-TOF-MS. (C) Core 1− and core 2-derived O-glycans in LS174T-Tn− and LS174T-Tn+ cells before and after transfection with WtCosmc or Mock (**P < 0.01, ***P < 0.001).
