Figure 2. Association of apoptosis and HFD in mice cerebral cortex at separate times. (A) Western blot analysis of cerebral cortex tissues of mice with HFD and chow diet controls (n = 3 to 4 per group). (B–G) Quantification of PARP (B), Bcl-2 (C), Bax (D), Bad (E), p21 (F), caspase3 (G). GAPDH as a loading control. Values are presented as means ± SD. *P < 0.05 and **P < 0.01 versus chow diet; one-way ANOVA by Tukey’s test.