Research Paper Volume 13, Issue 19 pp 22985—23003

Figure 3. FEYE builds a bridge between muscle and adipose. (A) FEYE can promote the mRNA expression of PGC1α and FNDC5 in skeletal muscle. Each experiment was performed in triplicate wells; (B) Western blot assay suggests that FEYE can promote the protein expression of PGC1α and FNDC5 in skeletal muscle. Each experiment was performed in triplicate wells; (C) ELISA assay shows that FEYE can significantly increase the expression of Irisin in serum. Each experiment was performed in triplicate wells; (D) Western blot analysis showed FEYE can significantly enhance the expression of UCP1 and PGC1α protein in inguinal adipose tissue (iWAT); (E) HE staining of inguinal adipose tissue (iWAT) and perirenal adipose tissue (pWAT) and immunohistochemical staining of UCP1 and PGC1α protein (red arrows indicate the yellow staining sites of UCP1, and blue arrows indicate the yellow staining sites of PGC1α) and analysis of the ratio of optical density to immunostaining area; (F–H) At room temperature, FEYE can promote heat production in mice (F) (n = 5/group), Each experiment was performed in quintuplicate wells. At 4°C, the body temperature of mice in the FEYE group is always higher than that in the sham group (G) (n = 3/group). In addition, in a cold environment, the temperature fluctuation is more stable in different time periods (H) (n = 3/group). (Bar = 100 μm); ^*P < 0.05, ^**P < 0.01.