Research Paper Volume 13, Issue 19 pp 22830—22842

Figure 2. Panaxadiol suppressed the proliferation of human pancreatic cancer cells. (A–B) The cell viability of PANC-1 and Patu8988 was detected by CCK8 assays. The IC50 values of panaxadiol for PANC-1 and Patu8988 cells were 22.0 μM and 48.9 μM correspondingly. PANC-1 and Patu8988 were treated with different concentrations of panaxadiol as soon as they were seeded in 96-well plates, and then they were incubated for 24 h. (C–D) Colony formation assays showed the proliferation of pancreatic cancer cells was suppressed. When the cells were plated in a 6-well plate and incubated for 24 h, 0, 20 μM, and 50 μM of panaxadiol was added to each well. After 14 days, the cells were fixed and stained with crystal purple to count the number of colonies. The results were presented as mean ± SD; ^*P < 0.05. (E) Ki67 immunofluorescence staining showed the Ki67 positive cells decreased after the treatment of panaxadiol.