Research Paper

Figure 3. Effect of SFB-derived exosomes like vesicles on inflammation and apoptosis in chondrocytes. (A) qRT-PCR analysis for relative mRNA expression levels of miR-214-3p in SFB transfected with miR-214-3p mimic after 24 hours of incubation. (B) Relative protein levels by western blot analysis for expression of CD9, HSP70 and CD63 in exosomes like vesicles. (C) CCK-8 analysis for cell proliferation after treatment with SFB-miR-214-3p-exo for 24 and 48 hours. (D) Apoptosis study using flow cytometer after treatment of SFb-miR-214-3p-exo for 2 hours before treatment of SNP for 24 hours. (E) qRT-PCR analysis for relative mRNA levels of miR-214-3p, IL-1β, IL-6 and TNF-α after treatment of SFB-miR-214-3p-exo for 2 hours prior to SNP treatment of 24 hours. (F) Western blot analysis for protein levels for expression of inflammatory proteins IL-1β, IL-6 and TNF-α after treatment of SFB-miR-214-3p-exo for 2 hours prior to SNP treatment of 24 hours. The results are presented as mean (n=3) ±%RSD.*P<0.05, **P<0.01 and ***P<0.001 compared to miR-control-exo, #P<0.05, ##P<0.01, ###P<0.001 compared to SNP+miR-Control-exo.