Research Paper Volume 13, Issue 18 pp 22528—22543

Figure 2. FSTL3 overexpression facilitated RCC proliferation and metastasis and cramped cell apoptosis. A498 and ACHN cells were transiently transfected along with FSTL3 overexpression plasmids. (A–C) qRT-PCR (A), Western Blot (B), and ELISA (C) were done for FSTL3 detection; (D, E) BrdU (D) and CCK8 (E) were implemented to examine cell proliferation; (F) Flow cytometry measured cell apoptosis; (G) Transwell evaluated cell invasion; (H) Western Blot assessed E-cadherin, Twist, and Slug expression in RCC cells. **P<0.01, ***P<0.001 (vs. the NC group). N=5.