Research Paper Volume 13, Issue 18 pp 22298—22314

Figure 4. Effect of AKR1B10 on the cell proliferation and migration ability of gastric cancer cells. (A) AKR1B10 expression in gastric cancer (GC) cell lines from the CCLE platform. (B) Relative expression of AKR1B10 in GC cell lines according to RNAseq results via the CCLE platform. (C–D) Immunoblot showing AKR1B10 protein levels in MKN45 cells transfected with AKR1B10-shRNA (C) and in AGS cells transfected with the AKR1B10 overexpression plasmid (D), and gray value analysis via ImageJ. (E–J) Proliferation rates (E–F), colony forming ability (G–H), and migration ability (I–J) of AKR1B10-KD and AKR1B10-OE GC cells. CCLE, Cancer Cell Line Encyclopedia. NC, negative control. KD, knockdown, AKR1B10-shRNA. VEC, vector. OE, overexpression, AKR1B10 overexpression plasmid. Data are presented as the mean ± SD (n = 3). ^*P < 0.05, ^**P < 0.01, ^***P < 0.001.