Research Paper Volume 13, Issue 18 pp 22286—22297

Figure 5. ASXL1 silencing sensitized ACC cells to etoposide, doxorubicin, cisplatin and mitotane (EDP-M) regimen. Xenograft murine models consisting of 8 male BALB/c nude mice per group undergoing EDP-M regimen () or vehicle control (Veh) with subcutaneous implanted with (A) NCI-H295R cells with or without ASXL1 silencing under left flank with tumor growth monitored over 60-day period and tumor size of < 2000 mm³ as endpoint, and (B) Kaplan-Meier curves of survival of mice; and with (C) SW-13 cells with or without ASXL1 silencing under left flank with tumor growth monitored over 60-day period and tumor size of < 2000 mm³ as endpoint, and (D) Kaplan-Meier curves of survival of mice; (E) Quantitative PCR showing expression of ASXL3 in ACC cell lines treated with EDP regimen; (F) Representative image of immunohistochemical staining of ASXL1 and ASXL3 in harvested tumors from xenograft models (scale bar = 100 μm, *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001).