Research Paper Volume 13, Issue 17 pp 21587—21598

Figure 2. FOXN3 suppresses glioma cell proliferation, survival and invasion. (A) FOXN3 protein expression levels in normal human L02 liver cells and glioma cell lines (SF126, SK-Hep-1, Huh6 and Huh7) were determined by Western blotting. (B) Transfection efficiency was detected via Western blotting after transfection with FOXN3 expression vectors in U87MG cells or transfection with specific shRNAs in SF126 cells. (C) Cell proliferation was measured by MTT assays after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. (D) Colony formation ability was analyzed using colony formation assays after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. (E) Apoptosis was assessed via flow cytometry after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. (F) Migration capability was evaluated by scratch wound healing assays after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. (G) Invasion ability was determined via Transwell invasion assays after transfection with FOXN3 expression vectors in U87MG cells or transfection with shRNA#1 in SF126 cells. ^**P < 0.01. FOXN3, forkhead box N3; shRNA#1, short hairpin RNA #1 specifically targeting FOXN3; shRNA#2, short hairpin RNA #2 specifically targeting FOXN3.