Research Paper Volume 13, Issue 16 pp 20534—20551

Figure 6. ROS generation was similar when NLRP3 inflammasomes were inhibited in the cardiocytes aging model. H9c2 cells were pre-treated with or without MCC950 (10μM), a commonly used NLRP3 inhibitor, for 1 hour, and then incubated with or without 10g/L D-gal for 24 hours. (A) ROS generation was detected using a DCFH-DA probe. Representative fluorescent images showed that ROS generation was similar no matter NLRP3 inflammasomes were inhibited or not in the cardiocytes aging model. (B) Intracellular ROS was quantified by flow cytometry.