Research Paper Volume 13, Issue 16 pp 20534—20551
NLRP3 inflammasome activation contributes to the pathogenesis of cardiocytes aging
Figure 3. ROS generation was increased in the cardiocytes aging model induced by D-gal. H9c2 cells were treated with different concentrations of D-gal (0, 2, 10 and 50 g/L) for 24 hours. (A) ROS generation was detected using a DCFH-DA probe. Representative fluorescent images showed that ROS generation was increased in the cardiocytes aging model induced by D-gal in vitro. (B) Intracellular ROS was quantified by flow cytometry.