Research Paper Volume 13, Issue 16 pp 20481—20494

Figure 6. miR-33a-5p inhibits the tumor growth of EC in vivo. (A–F) The effect of miR-33a-5p on tumor growth of EC cells in vivo was analyzed by nude mice tumorigenicity assay by injected with the TE-1 cells treated with control mimic or miR-33a-5p mimic. (A) Representative images of dissected tumors from nude mice were presented. (B) The average tumor volume was calculated and shown. (C) The average tumor weight was calculated and shown. (D) The expression levels of miR-33a-5p were measured by qPCR in the tumor tissues of the mice. (E) The protein expression of DKK1 was assessed by Western blot analysis in the tumor tissues of the mice. (F) Western blot of Cyclin D1, c-jun, CD44, and c-Met protein expression in TE-1 and Eca-109 cells and the protein bands. N = 5. The inter-group comparison was conducted by the independent sample t-test, multiple time point data were analyzed by repeated measures analysis of variance (ANOVA), mean ± SD, ^bP < 0.01.