Figure 5. DF-EVs regulate VEGFA expression through miR-195-5p and miR-205-5p. (A) Expression level of MiR-195-5p and miR-205-5p in HUVECs treated with DF-EVs or Control-EVs. (B, C) Expression level of VEGFA in HUVECs treated with DF-EVs or Control-EVs were detected by qRT-PCR and western blot separately. (D) The secretion of VEGFA in culture medium of HUVECs treated with DF-EVs or Control-EVs were detected by Elisa. *P < 0.05, **P < 0.01.