Research Paper Volume 13, Issue 17 pp 21134—21141
RNA N6-methyladenosine demethylase FTO promotes osteoporosis through demethylating Runx2 mRNA and inhibiting osteogenic differentiation
Figure 2. FTO over-expression impairs the osteogenic differentiation of BMSCs. (A) The efficiency of FTO over-expression in BMSCs were confirmed by quantitative real-time PCR. *P<0.05. (B) The efficiency of FTO over-expression in BMSCs were confirmed by western blot. *P<0.05. (C) The cell viability of BMSCs were analyzed by CCK8 assay. (D) Representative images and quantitative analyses of ALP and ARS staining of MSCs after 14 days of osteogenic induction. (E) qRT-PCR analyses of the expression of Runx2, Sp7, Alp and Bglap in MSCs after 7 days of osteogenic induction. *P<0.05.