Research Paper Volume 13, Issue 14 pp 18879—18893

Figure 6. Myeloid DJ-1 deficiency attenuates APAP induced hepatic inflammatory reaction via less ROS production and chemokines induction. Fasted DJ-1^fl/fl and Lysm-DJ-1^−/− mice were intraperitoneal injected with a single dose of 300 mg/kg of APAP. Quantification of Ly6G⁺CD11b⁺ neutrophils (A) and Ly6C⁺CD11b⁺ macrophages (B) by flow cytometry (n = 4). (C) Quantification of CXCL1 and CXCL2 expression in liver tissue 24 h after APAP challenge (n = 5–8). (D) Quantification of cellular ROS levels by oxidized DCFDA and flow cytometry in neutrophils and macrophages isolated from DJ-1^fl/fl and Lysm-DJ-1^−/− mice (n = 4). Data are shown as the means ± SD, ^*P < 0.05; ^**P < 0.01; ^***P < 0.001; ^****P < 0.0001.