Research Paper Volume 14, Issue 3 pp 1253—1264
Puerarin inhibits FUNDC1-mediated mitochondrial autophagy and CSE-induced apoptosis of human bronchial epithelial cells by activating the PI3K/AKT/mTOR signaling pathway
Figure 6. Puerarin inhibits mitochondrial autophagy and apoptosis in CSE-induced HBECs by activating the PI3K/AKT/mTOR signaling pathway. 3-MA, an inhibitor of PI3K, was added to 20% CES-induced HEBCs, which treated with puerarin at a concentration of 200 μg/mL. (A, B) The protein expression of total PI3K, p-PI3K, total AKT, p-AKT, total mTOR and p-mTOR in CSE-induced HBECs were analyzed by Western blotting. (C) Western blotting was used to detect the expression of mitochondrial autophagy-related proteins such as PINK1 and Parkin. (D) The protein expression of Cleaved caspase3 and Bax in CSE-induced HBECs were analyzed by Western blotting. β-actin was used as the loading control. N=6, ** P<0.01.