Research Paper Volume 13, Issue 14 pp 18586—18605

Figure 3. Expression of circSP3 in HCC tissues and cell lines. (A) CircSP3 levels in 48 pairs of HCC tissues and adjacent normal liver tissues were evaluated using qRT-PCR. (B) The relative circSP3 levels in four HCC cell lines (Hep-3B, Huh-7, Bel-7402 and SMMC-7721) and an immortalized liver cell line (HL-77O2) were determined using qRT-PCR. (C) CircSP3 levels in Hep-3B cells infected with NC or circSP3 plasmids. (D) qRT-PCR was conducted to confirm the knockdown efficiency of si-circSP3-1 and si-circSP3-2. (E) qRT-PCR was conducted to confirm the knockdown efficiency of LV3-circSP3. *p<0.05, **p<0.01 and ***p<0.001.