Research Paper Volume 13, Issue 14 pp 18527—18544

Figure 9. FRAT1 overexpression reversed the effects of CCAT1 suppression in PCa cells. (A) mRNA expression of FRAT1 in LnCaP and PC3 cells transfected with si-CCAT1 or si-CCAT1+pcDNA-FRAT1. (B) The results of MTT assays indicated that down-regulation of CCAT1 suppressed cell viability, and the suppressed ability was counteracted by pcDNA-FRAT1. (C) EdU staining results of LnCaP cells and PC3 cells treated with si-CCAT1 or si-CCAT1+pcDNA-FRAT1. Scale bar: 50 μm. (D) Flow cytometry detection of the cell apoptosis changes in LnCaP cells and PC3 cells treated with si-CCAT1 or si-CCAT1+pcDNA-FRAT1. (E, F) Cell migration and invasion were reduced with si-CCAT1 transfection, and recovered by overexpressing FRAT1. (G) Protein expression of FRAT1, E-cadherin, N-cadherin, and Vimentin in PCa cells were detected by western blot. *P < 0.05, **P < 0.01, compared with the si-NC group; #P < 0.05, ##P < 0.01, compared with the si-CCAT1 group.