Research Paper Volume 13, Issue 14 pp 18498—18514

Figure 5. M/T-Exo miRNA-205 affects breast cancer cells (BCCs) proliferation, migration, and invasion via targeting E2F1. (A–C) The abilities of colony formation, migration, and invasion in M/T-Exo-cocultured BCCs treated with the negative control miRNA-205 inhibitor (NCi), miRNA-205 inhibitor (205i), or the combination of 205i and lentiviral vector carrying E2F1 (E2F1). (D) The protein expression of Akt and the phosphorylation of Akt at Ser 473 (p-Akt Ser 473) in M/T-Exo-cocultured BCCs treated with the negative control miRNA-205 inhibitor (NCi), miRNA-205 inhibitor (205i), or the combination of 205i and lentiviral vector carrying E2F1 (E2F1). Values are means ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. At least three replicates were available for analysis in each treatment group.