Research Paper Volume 13, Issue 16 pp 20131—20148

Figure 3. Correlation of cytotoxicity of NKM cells and cell subpopulations. (A–D) NK cells (CD3⁻CD16⁺/CD56⁺) were positively correlated with the cytotoxicity of NKM cells, whereas T-helper cells (CD3⁺CD4⁺ T) were negatively correlated with the cytotoxicity of NKM cells. A total of 198 NKM cells was used for correlation analyses, and four subpopulations were assessed: NK cells (A), NK T-like cells (B), CD3⁺CD4⁺ T cells (C) and CD3⁺CD8⁺ T cells (D). Correlations and linear regressions were analyzed with Prism 7.04 (GraphPad). (E–G) CD3⁻CD16⁺CD56⁺ NK cells were correlated the cytotoxicity of NKM cells. A total of 44 NKM cells was used for correlation analyses, and four subpopulations were evaluated: CD56⁺CD16⁻ NK cells (E), CD56⁺CD16⁺ NK cells (F) and CD56⁻CD16⁺ NK cells (G). Correlations and linear regressions were analyzed with Prism 7.04 (GraphPad). (H) The cytotoxicity of NKM, NK+ and NK- cells. The NKM cells were separated into NK+ (CD56⁺ or CD16⁺ NK cells) and NK- (other non-NK cells) cells with immunomagnetic beads (NK Cell Isolation Kit). The ratio of effector cells: target cells was 10:1 or 20:1, and the incubation time was 2 h. The number on the upper right shows the cytotoxicity of effector cells.