Research Paper
Upregulation of miR-125b-5p relieves chondrocyte inflammation and apoptosis in osteoarthritis by repressing the YAP1/NF-κB pathway
Figure 6. Overexpressing miR-125b-5p alleviated the inflammatory response in OA rats. The OA rat model was constructed by destabilization of the medial meniscus (DMM). The knee tissues of OA rats were transfected with miR-NC as the control and further transfected with miR-125b-5p mimics. (A) qRT-PCR was utilized to compare the expression of COX-2, iNOS, TNF-α, and IL-6 in the knee tissues. (B) WB was employed to check the expression of COX-2 and iNOS in the knee tissues. (C) The Griess reaction method was employed to determine the NO content in each group in the knee tissues. (D) The concentration of TNF-α, IL-6 and PGE2 was calculated by ELISA in the knee tissues. (E, F) The protein expression of Collagen II, Aggrecan, ADAMTS5, MMP9, and YAP1/NF-κB in the knee tissues was compared by WB. ***P<0.001, NS P>0.05 (vs. OA group), ##P<0.01, ###P<0.001 vs. OA+miR-NC group, N=5.