Upregulation of miR-125b-5p relieves chondrocyte inflammation and apoptosis in osteoarthritis by repressing the YAP1/NF-κB pathway

Jianqiang Li; Zhijian Zhao; Kunfeng Chen; Yuhua Guo; Zhong Zhu; Lu Liang; Lingjun Jiang; Zhongyi Chen; Jiajing Ye; Daozhen Chen

doi:doi:10.18632/aging.203278

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Research Paper

Upregulation of miR-125b-5p relieves chondrocyte inflammation and apoptosis in osteoarthritis by repressing the YAP1/NF-κB pathway

Figure 5. Overexpressing miR-125b-5p alleviated OA development in rats. The OA rat model was constructed by destabilization of the medial meniscus (DMM). The knee tissues of OA rats were transfected with miR-NC as the control and further transfected with miR-125b-5p mimics. (A) Safranin O staining and toluidine blue staining were carried out to observe morphological differences of the rat knee sections. (B) The thickness (μM) of the subchondral bone plate. (C) OARSI score. (D) WB detected the expression of apoptotic proteins Bax, Bcl2 and Caspase3 in the knee tissues. (E) The expression of Bax, p-YAP1 and p-NF-κB was compared by IHC. ***P<0.001 (vs. sham group), NS P>0.05 (vs. OA group), ##P<0.01, ###P<0.001(vs. OA+miR-NC group). N=5.