Research Paper
Upregulation of miR-125b-5p relieves chondrocyte inflammation and apoptosis in osteoarthritis by repressing the YAP1/NF-κB pathway
Figure 4. Overexpressing miR-125b-5p played anti-inflammatory and anti-apoptotic roles by inhibiting the YAP1/NF-κB signaling pathway, thus alleviating OA. miR-125b-5p mimics were transfected into IL-1β (10 ng/mL)-induced ATDC5 cells, with 2 μg/mL Verteporfin treatment. (A) The miR-125b-5p expression was tested by qRT-PCR. (B) Cell viability was monitored by MTT assay. (C) Flow cytometry was conducted to monitor cell apoptosis. (D) WB was applied to determine the expression of Bax, Bcl2, and Caspase3. (E) The expression of COX-2, iNOS, TNF-α, and IL-6 was verified by qRT-PCR. (F–H) WB was implemented to examine the profiles of COX-2, iNOS, ECM-related markers (Collagen II, Aggrecan, ADAMTS5, and MMP9), and YAP1/NF-κB in ATDC5 cells.. *P<0.05, **P<0.01, ***P<0.001 (vs. IL-1β group), NS P>0.05 (vs. the IL-1β+miR-125b-5p group), N=3.