Upregulation of miR-125b-5p relieves chondrocyte inflammation and apoptosis in osteoarthritis by repressing the YAP1/NF-κB pathway

Jianqiang Li; Zhijian Zhao; Kunfeng Chen; Yuhua Guo; Zhong Zhu; Lu Liang; Lingjun Jiang; Zhongyi Chen; Jiajing Ye; Daozhen Chen

doi:doi:10.18632/aging.203278

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Research Paper

Upregulation of miR-125b-5p relieves chondrocyte inflammation and apoptosis in osteoarthritis by repressing the YAP1/NF-κB pathway

Figure 3. miR-125b-5p targeted YAP1. (A) The bioinformatics database Starbase was adopted to find out the potential target of miR-125b-5p. (B, C) Dual-luciferase reporter assay and RNA pull-down assay were employed to certain the targeted relationship between miR-125b-5p and YAP1. (D) WB was employed to define the YAP1/NF-κB level in ATDC5 cells transfected with miR-NC or miR-125b-5p mimics. NS P > 0.05, *** P < 0.001 (vs. miR-NC), *** P < 0.001 (vs Bio-NC group), N = 3.