Research Paper
Upregulation of miR-125b-5p relieves chondrocyte inflammation and apoptosis in osteoarthritis by repressing the YAP1/NF-κB pathway
Figure 2. Overexpressing miR-125b-5p eased IL-1β-induced chondrocyte injury. miR-125b-5p mimics were transfected into IL-1β (10 ng/mL)-induced ATDC5 cells to construct a miR-125b-5p overexpression model. (A, B) qRT-PCR was utilized to test the miR-125b-5p profile. (C) MTT assay was adopted to determine cell viability. (D) Flow cytometry was applied to detect cell apoptosis. (E) WB was implemented to compare the expression of Bax, Bcl2, and Caspase3. (F) qRT-PCR was adopted to determine the expression of COX-2, iNOS, TNF-α and IL-6 in ATDC5 cells. (G–I) WB was employed to measure the expression of inflammatory mediators (COX-2 and iNOS), ECM (Collagen II, Aggrecan, ADAMTS5, and MMP9), and YAP1/NF-κB in ATDC5 cells. *P<0.05, **P<0.01, ***P<0.001, #P<0.05, ##P<0.01, ###P<0.001, N=3.