Figure 4. Wnt ligands mRNAs were higher in EVs isolated from osteoporotic patients. Six mRNAs (Wnt1, Wnt5a, Wnt5b, Wnt7a, Wnt9a, and DKK) were selected for qRT-PCR experiments using eight osteoporotic patients compared with eight non- osteoporotic individuals. Data represent mean ± SEM from three independent experiments.