Research Paper Volume 13, Issue 17 pp 21090—21101

Figure 1. Notch1 expression and cell viability induced by doxycycline treatment. (A) The NICD1 expression induced by doxycycline treatment at various concentrations. GAPDH was used as loading control. *P<0.05, significantly different compared with the untreated control group. (B) Notch1 induced by doxycycline in various concentrations has inhibited cell viability in human osteosarcoma cell (MG-63). All the experiments were conducted in triplicates. (C) The NICD1 expression induced by doxycycline treatment at various time points including 0h, 12h, 24h, 48h, 36h and 60h. GAPDH was used as loading control. *P<0.05, significantly different compared with the untreated control group. (D) Notch1 induced by doxycycline in various time points including 0h, 12h, 24h, 48h, 36h and 60h has inhibited cell viability in human osteosarcoma cell (MG-63). All the experiments were conducted in triplicates.