Figure 3 — α-Cyperone (CYP) down-regulates NF-κB and MAPKs signaling, attenuating inflammation and extracellular matrix degradation in chondrocytes, to ameliorate osteoarthritis in mice

Research Paper|Volume 13, Issue 13|pp. 17690–17706

α-Cyperone (CYP) down-regulates NF-κB and MAPKs signaling, attenuating inflammation and extracellular matrix degradation in chondrocytes, to ameliorate osteoarthritis in mice

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Figure 3.Effects of CYP on IL-1β induced ECM degradation in chondrocytes. Levels of type II collagen, aggrecan, MMP-13 and ADAMTS5 were measured by ELISA (A). The type II collagen was assayed through cell immunofluorescence combined with DAPI staining for nuclei (scale bar:100 μm) (B). Expressions levels for type II collagen, ADAMTS5, MMP-13 and MMP-3 were detected by western blot assay (C) and quantified by Image Lab software (D). Data are presented as means ± S.D.## means p < 0.01 vs. the control group and ** p < 0.01, * p < 0.05 vs. the IL-1β alone group, n=5.