Research Paper Volume 13, Issue 13 pp 16957—16973

Figure 2. NMN increased TERT expression but reduced cellular senescence and myofibroblast differentiation. (A) BJ cells were treated with NMN, and then analyzed for TERT by qPCR. (B) BJ cells were treated with NMN followed by H₂O₂ induction, Cells collected at 6 hours or 48 hours after H₂O₂ treatment, were analyzed for CDKN2A and ACTA2 mRNAs by qPCR (left) or their respective protein expressions by western blotting (right). (C) Lung fibroblasts were transfected with 20 nM SIRT1 or control siRNA, and were analyzed for TERT and SIRT1 mRNA by qPCR. N = 5 in (A), in (B) and 4 in (C). ^*P < 0.05. ^**P < 0.01. ^****P < 0.0001.