Research Paper Volume 14, Issue 11 pp 4673—4698

Figure 2. Determining effects of CXCR4 knockdown on diminishing the cancer (EOC) proliferation capacity. CXCR4 expression in CXCR4-kD#1/OVCA420 (CXCR4 shRNA#1), CXCR4-kD#2/OVCA420 (CXCR4 shRNA#2) (A, B) and CXCR4/SKOV3 (CXCR4 cDNA) (F, G) cells were analysed by Western blotting (WB) and qRT-PCR, respectively. The cancer (EOC) proliferation capacity was determined by colony formation assay (C, H) with percentage of colony formation (D, I) in both OVCA420 and SKOV3 stable cell lines modified by CXCR4 knockdown or overexpression, respectively. The MTT assay was used to measure the proliferation of the CXCR4-shRNA knockdown OVCA420 (E) and CXCR4-overexpressed SKOV3 (J), respectively. Absorbance was measured at 490 nm using the average from triplicate wells. Data are presented as the mean ± SD of three independent experiments. Asterisk indicates P<0.05 compared with the controls as determined by t test.