Research Paper Volume 13, Issue 13 pp 17370—17379

Figure 6. BN treatment partially reverses GIO by suppressing AKT. (A) ELISA analysis shows phospho-AKT levels in GIO-induced MC3T3-E1 cells treated with BN (1μM, 10 μM, and 100 μM). (B–E) QRT-PCR analysis shows expression levels of bone turnover markers (ALP, OCN, Runx2, and Col 1) in GIO model MC3T3-E1 cells treated with BN (10 μM, 10 μM, and 100 μM BN). (F, G) ALP staining results of GIO model MC3T3-E1 cells treated with BN (1μM, 10 μM, and 100 μM). The data are represented as means±SD of at least 3 independent experiments.