miR-144-3p inhibited the growth, metastasis and epithelial-mesenchymal transition of colorectal adenocarcinoma by targeting ZEB1/2

Taiyuan Li; Cheng Tang; Zhixiang Huang; Lingling Yang; Hua Dai; Bo Tang; Benping Xiao; Jianfeng Li; Xiong Lei

doi:doi:10.18632/aging.203225

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Research Paper Volume 13, Issue 13 pp 17349—17369

miR-144-3p inhibited the growth, metastasis and epithelial-mesenchymal transition of colorectal adenocarcinoma by targeting ZEB1/2

Figure 6. ZEB1 and ZEB2 collaboratively abrogated the inhibitory effect of miR-144-3p on CRA cell proliferation and invasion. (A) qRT-PCR analysis of ZEB1, ZEB2 expression in Lovo^{miR-144-3p mimic} cells with overexpression of ZEB1 or/and ZEB2 and in HCT116^{miR-144-3p inhibitor} cells with knockdown of ZEB1 or/and ZEB2. (B) Western blot analysis of protein expression of ZEB1 and ZEB2 in indicated cells. (C) MTT assay of proliferation ability of Lovo^{miR-144-3p mimic} cells. (D) MTT assay of proliferation ability of HCT116^{miR-144-3p inhibitor} cells. (E) Transwell migration and invasion assays of the migration and invasion of Lovo^{miR-144-3p mimic} cells. (F) Transwell migration and invasion assay of HCT116^{miR-144-3p inhibitor} cells after knockdown of ZEB1 or/and ZEB2. *, P<0.05; **, P<0.01; ***, P < 0.001.