Research Paper Volume 13, Issue 12 pp 16763—16772

Figure 3. miR-30a-3p down-regulates APBB2. (A) DEmiRNA volcano map of the normal group and tumor group based on the GEO dataset. (B) Venn diagram of predicted down-regulated DEmiRNAs upstream of APBB2. (C) Pearson correlation analysis of APBB2 and miR-30a-3p. (D) The level of miR-30a-3p expression was down-regulated in the tumor group (red) compared with the normal samples (blue). (E) Box plots of miR-30a-3p expression associated with the different clinical stages of GA. (F) Survival curves associated with miR-30a-3p expression as an indicator of prognosis. Red indicates the high expression group and blue indicates the low expression group. (G) Expression of miR-30a-3p in BES-1 and GA cell lines (SGC-7901, MGC-803, and BGC-823). (H) Binding sites of miR-30a-3p and the 3′UTR of APBB2. (I and J) Results of the mRNA and protein levels were used to evaluate the effect of miR-30a-3p on APBB2. (K) A dual-luciferase reporter gene assay was used to determine the targeted binding of miR-30a-3p and APBB2. ^*P < 0.05.