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Research Paper Volume 13, Issue 12 pp 16471—16484
LncRNA HCG11/miR-579-3p/MDM2 axis modulates malignant biological properties in pancreatic carcinoma via Notch/Hes1 signaling pathway
Figure 5. HCG11, miR-579-3p and MDM2 co-regulated the biological behaviors of pancreatic carcinoma cells partly by regulating Notch/Hes1 pathway. (A) MDM2 protein expression AsPC-1 cells was detected by western blotting assay after upregulation of HCG11, HCG11+miR-579-3p, or HCG11+miR-579-3p+MDM2. (B–D) The OD values, cell cycle and clones number in AsPC-1 cells were analyzed by CCK-8, flow cytometry and colony formation assays when treatment with HCG11, HCG11+miR-579-3p, or HCG11+miR-579-3p+MDM2. (E) The apoptosis rates of AsPC-1 cells were determined by flow cytometry assay following HCG11, HCG11+miR-579-3p, or HCG11+miR-579-3p+MDM2 treatment. (F) The invaded number of AsPC-1 cells was estimated by Transwell chamber when treated by HCG11, HCG11+miR-579-3p, or HCG11+miR-579-3p+MDM2. (G, H) The protein expression levels of NICD and Hes1 in AsPC-1 cells were measured by western blotting assay after upregulation of HCG11, HCG11+miR-579-3p, or HCG11+miR-579-3p+MDM2. *p<0.05, **p<0.01 vs. pcDNA3.1, #p<0.05, ##p<0.01, ###p<0.001 vs. HCG11, &p<0.05, &&p<0.01 vs. HCG11+miR-579-3p.