Research Paper Volume 13, Issue 12 pp 16367—16380
High S100A9+ cell density predicts a poor prognosis in hepatocellular carcinoma patients after curative resection
Figure 6. Myeloid cells are the major source of S100A9. Multiple immunofluorescence staining shows DAPI (gray), S100A9 (green), CD15 (blue, A), and CD68 (red, D) expression and coexpression (double-positive cells) in the N and T regions. Quantification of CD15+ (B) and CD68+ (E) cell densities in the T and N regions (n = 12). (C) The percentages of S100A9+CD15+ cells among the total S100A9+ cells in the N and T regions. (F) The percentages of S100A9+CD68+ cells among the total S100A9+ cells in the N and T regions. (n = 12). Scale bar = 25 μm. N, nontumor, T, tumor. *P < 0.05, ****P < 0.0001, NS, no significance.