Research Paper Volume 13, Issue 12 pp 16009—16023

Figure 6. Calycosin inhibits breast cancer cell migration and invasiveness via BATF/TGFβ1. (A) RT-qPCR analysis shows relative levels of BATF, TGFβ1, CD147, MMP-2, and MMP-9 transcripts in BATF-overexpressing cells with or without TGFβ1 knockdown (left panel), and TGFβ1-overexpressing cells treated with or without calycosin (right panel). (B) Western blot analysis shows relative levels of BATF, TGFβ1, CD147, MMP-2, and MMP-9 proteins in BATF-overexpressing cells with or without TGFβ1 knockdown (left panel) and TGFβ1-overexpressing cells treated with or without calycosin (right panel). (C) Transwell invasion assay results show invasiveness of BATF-overexpressing cells with or without TGFβ1 knockdown (left panel) and TGFβ1-overexpressing cells treated with or without calycosin (right panel). (D) Wound healing assay results show the migration ability of BATF-overexpressing cells with or without TGFβ1 knockdown (left panel) and TGFβ1-overexpressing cells treated with or without calycosin (right panel). The data were represented as means ± SD. *P<0.05; **P<0.01; ***P<0.001.