Research Paper Volume 13, Issue 12 pp 16009—16023

Figure 1. Calycosin inhibits in vitro invasion and migration of breast cancer cells in a dose-dependent manner. (A) CCK-8 assay results show proliferation rates of control or calycosin-treated T47D and MCF-7 cells at 0, 24, 48, and 72 h. (B) RT-qPCR analysis shows relative levels of CD147, MMP-2, and MMP-9 transcripts in control or calycosin-treated T47D and MCF-7 cells. (C) Western blot analysis shows expression levels of CD147, MMP-2, and MMP-9 proteins in control or calycosin-treated T47D and MCF-7 cells. (D) Representative images show wound healing assay results in control or calycosin-treated T47D and MCF-7 cells at 0 h and 24 h. (E) Transwell invasion assay results show invasiveness of control or calycosin-treated T47D and MCF-7 cells. The data were represented as means ± SD. *P<0.05; **P<0.01; ***P<0.001.